USR PUBLISHER

This study evaluated the effect of ethanol extract of Zingiber officinale on chemokine levels, CCL2 – Monolyte Chemoattractant protein 1 (MCP-1), CCL5 - Rantes, and IL-8 (Inter leukin-8) in male Wistar albino rats with albumin-induced inflammation. Inflammation was experimentally induced by intraperitoneal injection of 0.5 ml of egg albumin solution in the hind paw region, and the animals were divided into five groups: blank control, negative control, standard control (treated with an anti-inflammatory drug), and two treatment groups administered low and high doses of Z. officinale extract. Chemokine levels were assessed to determine the extract's anti-inflammatory efficacy. The negative control group exhibited significantly elevated levels of CCL2 (227 ± 0.001 μg/L), CCL5 (143.60 ± 0.001 μg/L), and IL-8 (5.16 ± 0.003 pg/ml) compared to the blank control (p < 0.05), confirming inflammation. Treatment with Z. officinale extract resulted in a dose-dependent reduction in chemokine levels. The high-dose group notably decreased CCL2 to 128 ± 0.000 μg/L, CCL5 to 118.12 ± 0.003 μg/L, and IL-8 to 3.47 ± 0.041 pg/ml, with values statistically comparable to the blank and standard control groups (p > 0.05). In contrast, the low-dose group showed partial effectiveness, especially for IL-8 and CCL5. These findings indicated that Zingiber officinale possesses significant anti-inflammatory properties, effectively modulating chemokine expression and reducing inflammatory responses. The study supports its potential as a natural alternative for managing inflammation-related disorders.